To test whether SPR2 function is required for cortical array reorientation in response to blue light, we measured the rate of cortical array reorientation in etiolated hypocotyl cells of the spr2-2 mutant expressing mCherry-TUA5 to… HIV-1 viral protein u (Vpu) can stimulate novel versions of canonical interactions with the clathrin adaptor AP1 to counteract the host antiviral protein BST2. A light-dependent two-hybrid tool with transcriptional readout detects multiple protein-protein interactions in living mammalian cells with high signal-to-background ratios and enables genetic selections. Centromeric protein M (CENP-M) is required for the stabilization of a quaternary complex that plays a crucial role in kinetochore organization and function. We base this conclusion on the findings that the deletion of hym-1 resulted in COT1 activity that is reduced to a similar extent as described in Δpod-6 cells and that POD6-dependent Thr589 phosphorylation of COT1 is required for full…
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This result suggested Ii-FRB is an inefficient ER trapper for FKBP12-tagged proteins. We then reversed the configuration of the FRB and FKBP probes on EGFP-GPI and Ii, coexpressing Ii-FKBP12-mRFP and FRB-EGFP-GPI.
A comprehensive review of software programs used in biomedical research, based on Labome survey of formal publications. The software programs include those for image analysis, statistical analysis, flow cytometry, data analysis and graphing… Bound protein was washed with Buffer B (20 mM Hepes, pH 7.4, 1M NaCl, 25 mM Imidazole, 10% (vol/vol) glycerol, 6M urea, 6 mM βME) and eluted in Buffer C (20 mM Hepes, pH 7.4, 1M NaCl, 250 mM Imidazole, 10% (vol/vol) Glycerol, 6M Urea, 6 mM… Phosphorylation of ezrin involves an unusual mechanism requiring coincident binding to PIP2 and involvement of the non-catalytic domain to gain access to the phosphorylation site thereby restricting selective phosphorylation to the plasma… Tiles were stitched together using Slidebook 6.0 software to reconstruct a high-resolution image of bilateral striata. In each mouse, three sections at the following approximate coordinates were reconstructed (A-P distance from bregma): 1.7… The AAA-ATPase VCP sustains sarcoplasmic proteostasis, in part, by controlling autophagosome-lysosome fusion and the integrity of a dynamic tubular lysosomal network.
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15 Jun 2019 Download PDF Dilated cardiomyopathy (DCM) is one of the leading causes of heart controlled by SlideBook 6 software (Intelligent Imaging Innovations The Ca2+ transients were analyzed with Clampfit version 9.2 With the Clara, Andor delivers the highest sensitivity achievable a co-linear beam from up to six solid state lasers between A highly cost-effective USB 3.0 version is Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids Slidebook from III.
Phosphorylation of ezrin involves an unusual mechanism requiring coincident binding to PIP2 and involvement of the non-catalytic domain to gain access to the phosphorylation site thereby restricting selective phosphorylation to the plasma… Tiles were stitched together using Slidebook 6.0 software to reconstruct a high-resolution image of bilateral striata. In each mouse, three sections at the following approximate coordinates were reconstructed (A-P distance from bregma): 1.7… The AAA-ATPase VCP sustains sarcoplasmic proteostasis, in part, by controlling autophagosome-lysosome fusion and the integrity of a dynamic tubular lysosomal network. An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. NDR kinase Orb6 prevents RNP granule assembly and translational repression by promoting Sts5 association with 14-3-3 protein Rad24 to spatially control polarized cell growth. RAB-5 controls EFF-1 localization to the plasma membrane. a, Schematic of the PLM neuron in the tail of the animal; dashed box indicates the region depicted in b. b, Representative maximum projection confocal images of the PLM neuron in eff… Ch5_Flow - Free download as PDF File (.pdf), Text File (.txt) or read online for free. ce
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HIV-1 viral protein u (Vpu) can stimulate novel versions of canonical interactions with the clathrin adaptor AP1 to counteract the host antiviral protein BST2. A light-dependent two-hybrid tool with transcriptional readout detects multiple protein-protein interactions in living mammalian cells with high signal-to-background ratios and enables genetic selections.